The large Catholic church of Santiago Apóstol, also known as Santiago de los Caballeros, in Medina de Rioseco (Valladolid, Castilla y León, Spain), was built between the 16th and 17th centuries in an unusual mix of styles, combining very late Gothic with Plateresque, Classicist, and Herrerian Renaissance in the exterior and interior elevations, and Baroque in the vaulted roofs and monumental main altarpiece. It's a National Historic-Artistic Monument since 1964. The Ages of Man were held in Medina de Rioseco and Medina del Campo from May to November 2011.

Rodrigo Gil de Hontañón, a young architect, began building it in late Gothic style in 1533, during the Plateresque period. Later, he would design all his structures in Renaissance style. When the master died in 1577, the temple's enclosure at the church's base was unfinished. Thereafter, Valladolid Cathedral architect Alonso de Tolosa, who followed the Herrerian Renaissance style, constructed the major west façade. Juan de Hermosa, a reliable and experienced Medina de Rioseco worker, executed it.