The Diocesan Museum of Huesca is located in the cathedral square, in the historic centre of Huesca, and depends on the bishopric of Huesca. Part of the museum is located in the cathedral itself.

It was founded in 1945, by decree of Bishop Lino Rodrigo, and its structure was renovated in four chronological phases, from 1997 to 2004.

It was closed in 1969, due to restoration work on the Cathedral, and in 1975 it was reopened as the Diocesan Museum by Bishop Javier Osés.

Between 1997 and 2001 it was remodelled with a project by the architect Joaquín Naval, and a programme by the historian of artistic heritage and former director of the museum, Antonio Naval.

It is divided into four rooms, where sacred art is represented in the following sections: goldsmithery, Romanesque, Gothic and Renaissance-Baroque.

The goldsmiths' room is divided into two parts: one in the chapterhouse anteroom and the other in the chapterhouse. This section features goldsmith's work from the Middle Ages onwards. Three Romanesque chests from the Limoges workshop and a silver predella from 1367 in the Italo-Gothic style stand out. The chapterhouse offers silver works from the 16th to 18th centuries, processional crosses, chalices, cruets, cruets and also medieval codices from the cathedral, printed books from the first Aragonese printers and 16th century choir books.

The Romanesque room is divided into two cloisters: the Romanesque cloister and the cloister of Pope Luna. On the walls of the Romanesque cloister are preserved medieval burials and tombstones with inscriptions of some Aragonese nobleman or ecclesiastic from the 12th to the 14th century. Inside this cloister there are different mural paintings and polychrome carvings from different parts of the diocese. In the cloister of Pope Luna there are Gothic paintings from the 15th century.

The Renaissance-Baroque room begins with the main altarpiece from the 16th century, commissioned by the Abbot of Montearagón and the Archbishop of Zaragoza by the sculptor Gil de Morlanes el Viejo. There are also Flemish-influenced paintings from the same century in the room. The next room shows a silver altar from the 18th century representing saints from Huesca. There is also an altarpiece from the 16th century and several pieces from the same century. Baroque carvings from the 18th century are on display. Finally, the choir stalls of the cathedral, sculpted between 1577 and 1591, is made up of 55 chairs but formerly had 85.